FIG. 7.

Quantification of angiogenesis as a function of scaffold depth at day 7. One million EPCs were seeded in each scaffold. The human CD31-positive vessels in scaffold sections were outlined with red, blue, and green color for 0–200, 200–400, and 400–600 μm intervals, respectively (A). The vessel density and percentage of area occupied by vessels (B) were evaluated at 200-μm intervals. Values represent the mean and standard deviation (n=3). V0 and V10 represent scaffolds without VEGF and loaded with 10 μg of VEGF, respectively. H0 and Hm represent scaffolds without and with cross-linked heparin, respectively. Scale bar=500 μm. Color images available online at www.liebertonline.com/tea