Figure 2.

Zeta potential and particle sizer. A) The nanoparticles were prepared by adjusting the pH of the human serum albumin solution to 8.0 and controlling the amount of coacervation agent to form nanoparticles of size approximately 101.0 ± 0.9 nm. B) The protein nanoparticles were negatively charged and the zeta potential was −18 ± 2.9 mV at pH 7.0. The zeta potential of the glutaraldehyde crosslinked nanoparticles decreases when the concentration of glutaraldehyde is increased. Zeta potential measurements were performed in phosphate buffer at pH 7.0 because pH and ionic strength of the dilution medium affects the magnitude of the zeta potential. The polydispersity index was found to be 0.3.