Figure 3.

The cytotoxicity of the blank nanoparticles crosslinked with a series of glutaraldehyde concentrations was studied by incubating HUVECs for 96 hours with the nanoparticles. Percent cell viability over initial number of HUVECs treated with nanoparticles for up to 96 hours is shown. MTS assay was used to determine the cell viability after exposure to the nanoparticles. Nanoparticles without glutaraldehyde coating showed a cell viability of above 90% after 96 hours. Increasing the amount of glutaraldehyde in the particle preparation increased the cytotoxicity of the nanoparticles to the seeded HUVECs.

Abbreviation: HUVECs, human vascular enthothelial cells.