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Purification of lysin. (A) Silver-stained SDS/PAGE of purified lysin. Molecular mass of Kaleidoscope (Bio-Rad) standards is given in kilodaltons. (B) A bacterial lawn of group A streptococci D471 treated with a buffer control disk (1) or a disk containing 250 units of purified lysin (2), as described in Materials and Methods. A clearing zone indicates antibacterial activity.
