Table 4.
Pretreatment of mice with lysin (250 units) prevents streptococcal infections
| Lysin
treated
|
Buffer control
|
||||
|---|---|---|---|---|---|
| Mouse | Day 1 | Day 2 | Mouse | Day 1 | Day 2 |
| Crude
lysin | |||||
| 1 | 0 | 0 | 1 | 1 | 14 |
| 2 | 0 | 0 | 2 | 33 | 250 |
| 3 | 9 | 0 | 3 | 0 | 0 |
| 4 | >300 | >300 | 4 | 0 | 0 |
| 5 | 1 | 0 | 5 | 4 | 12 |
| 6 | 0 | 0 | 6 | 1 | 2 |
| 7 | 0 | 0 | 7 | 6 | >300 |
| 8 | 0 | 0 | 8 | 6 | 0 |
| 9 | 0 | 0 | 9 | >300 | Dead |
| 10 | 0 | 0 | 10 | 83 | >300 |
| Purified
lysin |
11 | 10 | >300 | ||
| 11 | 0 | 0 | 12 | 0 | 0 |
| 12 | 1 | 0 | 13 | 0 | n.d. |
| 13 | 0 | 0 | 14 | 150 | n.d. |
| 14 | 1 | 1* | 15 | 0 | n.d. |
| 15 | 11 | 10* | 16 | >300 | n.d. |
| 16 | 0 | 0 | 17 | 200 | n.d. |
| 17 | 0 | n.d. | |||
| 18 | 0 | n.d. | |||
| 19 | 0 | n.d. | |||
| 20 | 0 | n.d. | |||
| 21 | 0 | n.d. | |||
| Total | 6/21 | 3/16 | Total | 12/17 | 8/12 |
n.d., no data collected; indicated numbers are cfus.
*
Isolated streptococci remained sensitive to lysin treatment in vitro.