Figure 1.

The expected number of different alleles for two-, three-, and four-person mixtures. Assuming no allele dropout, the expected numbers of different alleles for the mixtures tested in this study were enumerated. Since the DNA of the individual donors was combined with various ratios and template amounts, the total number of combinations was 206 where n = 27 for two-person mixtures (gray columns), n = 105 for three-person mixtures (black columns), and n = 43 for four-person (gray columns with black outline) mixtures.