Figure 1.

First round polymerase chain reaction (frPCR) primer design. The squares represent the primer binding sequence, the circles represent the multiplex identifier (MID) sequence, and the thick line represents the fusion primer sequence for 454 applications. The length of each primer is 49 nucleotides. The total amplicon length is 503 bps; 49 nucleotides of forward and reverse primers +405 bps of hypervariable segment 1 (HV1) representing target sequence between nucleotide positions 15 997 and 16 401.