Figure 2.

Heteroplasmy at positions 16 093, 16 261, and 16 311 of sample F2, as observed when using the Sanger method of DNA sequencing. The only position where heteroplasmy would have been called by the majority of forensic scientists is 16 311; laboratories using instruments with low levels of signal to noise may have called position 16 093. The corresponding percentages of heteroplasmy observed in the second generation sequencing data were, 3.71%, 1.29%, and 20.14%, respectively.