Figure 4.

A chimeric mitochondrial DNA sequence for a sample from a 1:100 mixture of M15 and F22. The second sequence from the top has the 16069 T, 16126 C, and 16222 T polymorphisms of the minor component; highlighted by the NextGENe software. The second sequence from the bottom is a chimeric product that contains the 16 069 T and 16 126 C from the minor component, and the 16 224 C and 16 311 C polymorphisms from the major component. This is referred to as a Minor Front, Major Back chimeric sequence. The missing C’s at position 16 224 for the 4th, 5th, and 7th sequences listed below are indicative of the difficulties of the 454 approach to resolve homopolymeric stretches of greater than 4 C’s in a row.