Table 1.

First round polymerase chain reaction (frPCR) primer sequences for hypervariable segment 1. Starting at the 5′-end of each primer, fusion primer sequence for forward and reverse emulsion PCR and pyrosequencing is in standard font, followed by multiplex identifier sequence in italics and primer binding sequence in bold

Primer	Sequence
MID1 forward	5′ – GCCTCCCTCGCGCCATCAG ACGAGTGCGTCACCATTAG CACCCAAAGCT – 3′
MID1 reverse	3′ – GTGGTAGGAGGCACTTTAGTTGCGTGAGCA GACTCG CCCGACCGTTCCG – 5′
MID9 forward	5′ - GCCTCCCTCGCGCCATCAG TAGTATCAGCCACCATTAG CACCCAAAGCT – 3′
MID9 reverse	3′ – GTGGTAGGAGGCACTTTAGTCGACTATGAT GACTCG CCCGACCGTTCCG – 5′
MID10 forward	5′ - GCCTCCCTCGCGCCATCAG TCTCTATGCGCACCATTAG CACCCAAAGCT – 3′
MID10 reverse	3′ – GTGGTAGGAGGCACTTTAGTGCGTATCTCT GACTCG CCCGACCGTTCCG – 5′
MID11 forward	5′ – GCCTCCCTCGCGCCATCAG TGATACGTCTCACCATTAG CACCCAAAGCT – 3′
MID11 reverse	3′ – GTGGTAGGAGGCACTTTAGTTCTGCATAGT GACTCG CCCGACCGTTCCG – 5′
MID12 forward	5′ – GCCTCCCTCGCGCCATCAG TACTGAGCTACACCATTAG CACCCAAAGCT – 3′
MID12 reverse	3′ – GTGGTAGGAGGCACTTTAGTATCGAGTCAT GACTCG CCCGACCGTTCCG – 5′