Table 2.

First round polymerase chain reaction (frPCR) primer sequences for primer set two (PS2) of hypervariable segment 1, the D18S51 short tandem repeat locus, and the DYS389 I/II loci. Starting at the 5′-end of each primer, fusion primer sequence for emulsion PCR and pyrosequencing is in standard font, followed by multiplex identifier sequences in italics and primer binding sequence in bold

Primer	Sequence
PS2 MID1 forward	5′ – GCCTCCCTCGCGCCATCAG ACGAGTGCGTATACTTGA CCACCTGTAGTAC – 3′
PS2 MID1 reverse	3′ – GTGGTAGGAGGCACTTTAGTTGCGTGAGCA GACTCG CCCGACCGTTCCG – 5′
PS2 MID11 forward	5′ – GCCTCCCTCGCGCCATCAG TGATACGTCTATACTTGA CCACCTGTAGTAC – 3′
PS2 MID11 reverse	3′ – GTGGTAGGAGGCACTTTAGTTCTGCATAGT GACTCG CCCGACCGTTCCG – 5′
D18S51 MID1 forward	5′ – GCCTCCCTCGCGCCATCAG ACGAGTGCGTTGACAAA TTGAGACCTGTCTC – 3′
D18S51 MID1 reverse	3′ – GAGGTGTGTGGTCTCTTCAATGCGTGAGCA GACTCG CCCGACCGTTCCG – 5′
D18S51 MID11 forward	5′ – GCCTCCCTCGCGCCATCAG TGATACGTCTTGACAAA TTGAGACCTGTCTC– 3′
D18S51 MID11 reverse	3′ – GAGGTGTGTGGTCTCTTCAATCTGCATAGT GACTCG CCCGACCGTTCCG – 5′
DYS389 I/II MID1 forward	5′ – GCCTCCCTCGCGCCATCAG ACGAGTGCGTAACTCTCA TCTGTATTATCTATG – 3′
DYS389 I/II MID1 reverse	3′ – CCCTATTAACACAAGGAGTTCTGCGTGAGCA GACTCG CCCGACCGTTCCG – 5′
DYS389 I/II MID11 forward	5′ – GCCTCCCTCGCGCCATCAG TGATACGTCTAACTCTCA TCTGTATTATCTATG– 3′
DYS389 I/II MID11 reverse	3′ – CCCTATTAACACAAGGAGTTCTCTGCATAGT GACTCG CCCGACCGTTCCG – 5′