Figure 6.

Reduced in vitro differentiation of NF1^Prx1 satellite cells. (A) RT–PCR shows efficient recombination of the Nf1flox allele in Nf1^Prx1 satellite cells. (B) Three-day cultures were stained for Pax7 (marks muscle satellite cells) and EdU for proliferating cells. (C) Quantification of Pax7 cells relative to total cell number (DAPI). (D) Proliferating cells relative to total cell number (DAPI) and relative to Pax7⁺ cell number. (E) Muscle differentiation: 3 days after myogenic induction, myotube differentiation was analyzed with antibodies against meromyosin MF-20. (F) Quantification of the nuclei within MF20-positive area versus total nuclei (fusion index) shows reduced myogenesis in Nf1^Prx1 satellite cells.