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. 2011 Jun 20;6(6):e21280. doi: 10.1371/journal.pone.0021280

Table 1. 16S rDNA gene amplification parameters for qPCR assay from activated wastewater.

qPCR Assay Targetsa Amplification Parameters
Amplification efficiency ± SD Slope ± SD y intercept ± SD R2 Standard Range
General Bacteria 1.02±0.025 −3.29±0.056 37.7±0.80 >.98 103–108
Division TM7 0.96±. 0.019 −3.44±0.051 36.0±0.48 >.99 102–107
TM7a-like 0.92±0.020 −3.53±0.056 35.5±0.65 >.99 101–106
a

Each assay target consisted of three triplicate runs, total sample population (n = 9).