Table 1. 16S rDNA gene amplification parameters for qPCR assay from activated wastewater.
qPCR Assay Targetsa | Amplification Parameters | ||||
Amplification efficiency ± SD | Slope ± SD | y intercept ± SD | R2 | Standard Range | |
General Bacteria | 1.02±0.025 | −3.29±0.056 | 37.7±0.80 | >.98 | 103–108 |
Division TM7 | 0.96±. 0.019 | −3.44±0.051 | 36.0±0.48 | >.99 | 102–107 |
TM7a-like | 0.92±0.020 | −3.53±0.056 | 35.5±0.65 | >.99 | 101–106 |
Each assay target consisted of three triplicate runs, total sample population (n = 9).