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. 2011 Jun 20;6(6):e21172. doi: 10.1371/journal.pone.0021172

Figure 2. Dia2 is required for regulation of transcription.

Figure 2

2A) Dia is required of basal expression and repression of histone H2A/H2B genes Total RNA was extracted from yeast cells grown in YPD either in the absence (asyn) or after addition of 200 mM of hydroxyurea (HU) for 40 minutes to induce repression. RNA was reverse transcribed and analysed by quantitative PCR. Samples were normalised against expression of the ACT1 gene. 2B) Efficient induction of GAL1 expression requires Dia2 Yeast cells grown exponentially in raffinose were induced for GAL1 expression by the addition of galactose. Samples were taken at various time intervals for RNA extraction followed by qPCR. Signals from triplicate samples were amplified using GAL1 primers and normalised against those obtained using ACT1 primers. Wild-type (WT) cells showed a rapid increase in GAL1 signal from 15 minutes. This peaked at around 60 minutes. In Δdia2 cells, GAL1 expression did not increase until 20 minutes, and the observed maximal level of induction was about half of that achieved in wild-type cells.