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. 2011 Jun 20;6(6):e21172. doi: 10.1371/journal.pone.0021172

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Andress et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Yeast cells grown exponentially in raffinose were induced for GAL1 expression by the addition of galactose. Samples were taken at various time intervals for RNA extraction followed by qPCR. Signals from triplicate samples were amplified using GAL1 primers and normalised against those obtained using ACT1 primers. Unlike wild-type cells, Δhtl1 and Δrtt102 cells were defective in GAL1 expression similar to Δdia2 cells. This was most evident at 25 minutes. The observed steady state at 120 minutes was about half of that achieved in wild-type cells in all of the mutants.