Fig. 1.

Expression of HSP70 mRNA in porcine ovarian granulosa cells cultured in conditions of normal (37.5°C) or high (41.5°C) temperature, with and without serum and with and without hormones IGF-I, leptin and FSH. Values are means ± SED. a Effect of hormone treatment: significant (p < 0.05) differences between control cells (cultured without hormones) and cells cultured with hormones in similar conditions, b effect of high temperature: significant (p < 0.05) differences between corresponding groups of cells cultured in conditions of normal and high temperature, c effect of serum deprivation: significant (p < 0.05) differences between corresponding groups of cells cultured with and without serum. Data of RT-PCR