Table 1.
Experimental design
| Series no. | Parameters of experiments | |||
|---|---|---|---|---|
| Object and method of analysis | Temperature (°C) | Presence of serum | Addition of hormones | |
| 1 | HSP70, HSP72 and HSP105 mRNA in ovarian granulosa cells, RT-PCR | 37.5 | Serum | None |
| IGF-I | ||||
| Leptin | ||||
| FSH | ||||
| 41.5 | Serum | None | ||
| IGF-I | ||||
| Leptin | ||||
| FSH | ||||
| 37.5 | No serum | None | ||
| IGF-I | ||||
| Leptin | ||||
| FSH | ||||
| 41.5 | No serum | None | ||
| IGF-I | ||||
| Leptin | ||||
| FSH | ||||
| 2 | HSP70 in ovarian granulosa cells and ovarian follicles, SDS-PAGE–Western blotting | 37.5 | Serum | None |
| IGF-I | ||||
| Leptin | ||||
| FSH | ||||
| 41.5 | Serum | None | ||
| IGF-I | ||||
| Leptin | ||||
| FSH | ||||
| 37.5 | No serum | None | ||
| IGF-I | ||||
| Leptin | ||||
| FSH | ||||