Table 1.
AChE a | BChE | |||
---|---|---|---|---|
Enzyme form | E | E′ | E | E′ |
ki (M−1min−1) 10% MeOH | >3.7×106(b) | (1.06±0.23)×105 | (1.5±0.2)×108 | (2.5±0.7)×107 |
ki (M−1min−1) 5% acetonitrile | ND | (0.33±0.17)×105 | ND | ND |
ki (M−1min−1) 0.9% saline | ND | (0.73±0.6)×105 | ND | ND |
E, enzyme fast forms; E′ enzyme slow forms
ND, not determined
Experiments were performed in triplicate (mean values ± SE)
experimental ki values for the E-form of AChE are not listed because the kinetics did not provide a second order rate constant in that instance.
estimated minimum ki value using kp= 0.37 min−1 and KI≪0.1 μM