Table 1.

Bimolecular rate constants (k_i) for inhibition of human AChE and BChE at pH 8.0 and 25°C.

	AChE a		BChE
Enzyme form	E	E′	E	E′
ki (M−1min−1) 10% MeOH	>3.7×106(b)	(1.06±0.23)×105	(1.5±0.2)×108	(2.5±0.7)×107
ki (M−1min−1) 5% acetonitrile	ND	(0.33±0.17)×105	ND	ND
ki (M−1min−1) 0.9% saline	ND	(0.73±0.6)×105	ND	ND

E, enzyme fast forms; E′ enzyme slow forms

ND, not determined

Experiments were performed in triplicate (mean values ± SE)

^aexperimental k_i values for the E-form of AChE are not listed because the kinetics did not provide a second order rate constant in that instance.

^bestimated minimum k_i value using k_p= 0.37 min⁻¹ and K_I≪0.1 μM