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. Author manuscript; available in PMC: 2012 Jul 15.
Published in final edited form as: Free Radic Biol Med. 2011 Apr 17;51(2):406–416. doi: 10.1016/j.freeradbiomed.2011.04.024

Figure 6. Oxidative stress is induced in kidney-specific MnSOD KO mice.

Figure 6

Increased nitrotyrosine protein expression was detected in the tubules of 100% KO (c, g, k) and 50% KO (b, f, j) mice when compared to Kidney Cre mice (a, e, i). The specificity of nitrotyrosine antibody binding in the renal tissue was confirmed by blocking the antibody with 3′nitrotyrosine (10 mM) using 100% KO mice kidney sections (d, h, l). Representative micrographs of 400× magnification are shown. Bar indicates 50 μm. (B) Expression level of nitrotyrosine was evaluated and scored (Kidney Cre vs. 100% KO, P = 0.0008 and Kidney Cre vs. 50 % KO, P = 0.0012). Error bar indicates Mean ± SEM (n = 6).