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. 2009 Jan;18(1):37–45. doi: 10.1089/scd.2007.0255

FIG. 2.

FIG. 2.

Detection of transketolase from BM-derived IPCs. (A) RT-PCR analysis for expression of transketolase and insulin I genes in INS-1 cells, freshly isolated BM-derived cells (D0), cultured BM-derived cells (D1 to D13). (B) Effect of benfotiamine or oxythiamine on INS-1 cells proliferation using MTT assay. To determine the level of cell proliferation, the INS-1 cells were cultured in INS-1 culture medium, with benfotiamine (1 mM, 100 and 10 μM) or oxythiamine (10 and 1 μM). The MTT assay was performed on cells cultured for 24 and 48 h. Data represent the mean ± SD of five independent experiments. *p < 0.05 and **p < 0.01 compared with each experiment of glucose concentration. (C) Test of glucose levels in INS-1 cells culture-conditioned media. The samples were collected from the INS-1 cells cultured in high-glucose (4,500 mg/dL) medium, with benfotiamine (1 mM, 100 and 10 μM) or oxythiamine (10 and 1 μM). The cells were cultured for 5 h after which culture-conditioned media was collected and assayed for glucose concentration in media. The dotted line indicated basal glucose concentration (4,500 mg/dL). Data represent the mean ± SD of five independent experiments. a–dMeans within columns with different superscripts are different (at least p < 0.05).