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. 2001 Mar 27;98(7):4119–4124. doi: 10.1073/pnas.051004198

Figure 3.

Figure 3

Methylation status in the Lyta promoter region. (A) Sequencing image shows the bisulfite modified sequence. All of the cytosines except for methyl-cytosine were converted to thymine when treated with bisulfite. Arrows indicate the CpG sites. Numbers indicate the position relative to the Lyta transcription start site. (B) Methylation status in the Lyta promoter region of BCBL-1 cells untreated or treated with TPA for 24 h. Each included six independent clones. Solid circles indicate methylated CpG, and open circles indicate demethylated CpG. (C) Time course of TPA treatment. DNA samples from BCBL-1 cells treated with TPA at time intervals of 1, 4, 8, 12, and 24 h were bisulfite-modified, PCR-amplified, and sequenced directly without the separation of a single clone. Arrows show the CpG sites, which were mixed with C (methylated C) and T (demethylated C). T, A, G, and C are shown as red, green, black, and blue, respectively, in the Applied Biosystems sequencing image.