Figure 1.

Inhibition of PC-3 colony formation by peptide P111-136 and association with the HARP receptors. (A), Western blot analysis of HARP in the conditioned media of PC-3 cells (PC-3), 20 ng of recombinant HARP was used as reference. (B), polyclonal anti-human HARP antibody or control IgG or (C), P111-136 peptide were added to the culture medium twice a week. After 12 days, colonies with diameters greater than 50 μm were identified using a phase-contrast microscope equipped with a reticule. Representative pictures of colonies are shown below each treatment. Scale bar, 100 μm. Asterisks denote statistically significant differences with the corresponding untreated cells. Data are the means +/- SD of two experiments, each carried out in triplicate. *0.01 <p < 0.1, **0.001 <p < 0.01, and *** p < 0.001.