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. 2011 May 19;8:241. doi: 10.1186/1743-422X-8-241

Figure 2.

Figure 2

SDS-PAGE of the purified fusion protein and rabbit anti-His6-tagged gI IgG and Western blotting analysis. A. Induction of the His6-tagged gI fusion protien in E. coli. Recombinant plasmid pET-32a(+)-gI was transformed into bacteria. Lane M, molecular mass markers (in kDa); lane 1, protein products of the uninduced recombinant bateria; lane 2, protein products of the induced recombinant bateria; lane3, purified fusion protein His6-tagged gI. The arrowhead indicates the induced gI fusion protein; B. SDS-PAGE analysis of the purified rabbit anti-His6-tagged gI IgG. Lane M, protein molecular weight marker(in kDa); lane 1, rabbit anti-His6-tagged gI IgG obtained by ammonium sulfate precipitation; lane 2, rabbit anti-His6-tagged gI IgG obtained by High-Q anion exchange chromatography; C. Reactivity and specificity of the purified gI antiserum analyzed by Western blotting. Proteins were separated by SDS-PAGE and transferred to PVDF membranes. Lane M, protein molecular weight marker(in kDa); lane 1, the membranes were incubated with the gI antiserum; lane 2, the membranes were incubated with preimmune rabbit serum. Arrowheads indicate the fusion protein His6-tagged gI.