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Determination of mRNA expression of gI in DEV-infected DEFs. The total RNA were harvested from uninfected(Neg) or DEV-infected DEFs at different times p.i. (0.5, 1, 1.5, 2, 3, 4, 6, 8, 12, 24, 36, 48, and 60 h), presented on the x-axis. After reverse transcription, the cDNA was used as template for SYBR Green RT-PCR analysis, the data were normalized to the expression level of β-actin, as indicated in the y-axis. Data were analyzed using the iQ5 optical system software (Bio-Rad)
