Fig. 6.
Arcuate nucleus (ARH) kisspeptin (Kiss1) and neurokinin B (NKB) immunohistochemistry during dioestrus and lactation. Immunohistochemistry for Kiss1 (A, B) and NKB (D, E) revealed decreases in ARH Kiss1-immunoreactive (ir) positive cells in lactating animals (n = 4) compared to dioestrus virgin controls (n = 4, E). ARH NKB-ir positive cell bodies were also decreased in intact lactating animals (n = 4) compared to dioestrus virgin controls (n = 3, F). Cell numbers were counted as the total number of cells observed in four sections per animal. Scale bars = 50 μm; *P < 0.05.