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Restriction enzyme maps of pYT-1 and domain structure of the PelA polypeptide. Thin lines correspond to vector plasmid DNA. pYT-1 contains the entire 6.7-kb EcoRI fragment. The pectate lyase gene, pelA, was located on a 3.7-kb fragment designated as pYT-2. The white arrows indicate the coding sequences for the PelA, EngY, and HelA polypeptides, respectively. The different domains of PelA are named and filled with different patterns. PL1, pectate lyase family 1; CD, catalytic domain.
