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(A) Homotrimeric DdBrk1 maintains a dynamic pool of monomers, (B) which immediately associate with nascent Scar/WAVE and Abi proteins to form heterotrimeric subcomplexes. (C) Immediately thereafter, Scar/Abi/Brk1 heterotrimers are captured by the heterodimeric Nap/Pir platform to form the mature Scar/WAVE-complex (D).
