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. Author manuscript; available in PMC: 2012 Jun 10.
Published in final edited form as: DNA Repair (Amst). 2011 May 4;10(6):586–594. doi: 10.1016/j.dnarep.2011.03.006

Figure 1. Purified Mei5-Sae3, Sae3, RPA, Rad52, and MBP-Mei5 proteins and stoichiometry of the Mei5-Sae3 complex.

Figure 1

Mei5-Sae3 complex (1.5 μg; A, lane 1), Sae3-(HIS)6 (1.5 μg; A, lane 2), RPA (3 μg; B), Rad52 (1 μg; C, lane 1) and MBP-Mei5 (1 μg; C, lane 2) were resolved by 12% SDS-PAGE polyacrylamide gel and stained with Coomassie Blue. (*) Indicates the C-terminal truncation product of MBP-Mei5. The Mei5-Sae3 complex (D) was filtered through a 20 mL Sephacryl S-100 column. Fractions were TCA precipitated and subjected to SDS-PAGE on 12% polyacrylamide gels and stained with Coomassie Blue. Protein standards (Bio-Rad) bovine thyroglobulin (670 kDa, Void), bovine γ-globulin (158 kDa), chicken ovalbumin (44 kDa), horse myoglobin (17 kDa) and vitamin B12 (1.35 kDa) were filtered on a 20 mL Sephacryl S-100 column and eluted in the indicated fractions.

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