Table 2.
Conversion of 14,15-cis-EET to 14,15-threo-DHET and 14,15-trans-EET to 14,15-erythro-DHET by RBCs of vehicle- and AUCB-treated WKY and SHR
| DHET Produced, pmol · min−1 · 10−9 RBCs |
||
|---|---|---|
| Rat RBCs | 14,15-threo- | 14,15-erythro- |
| WKY vehicle | 2.82 ± 0.34 | 4.37 ± 0.52# |
| WKY AUCB | 1.67 ± 0.20* | 1.84 ± 0.22* |
| SHR vehicle | 4.40 ± 0.53 | 7.02 ± 0.70# |
| SHR AUCB | 2.59 ± 0.31* | 2.81 ± 0.34* |
Values are means ± SE; , n = 6-8 rats in each group. DHET, dihydroxyeicosatrienoic acid; RBC, red blood cell. 14,15-cis- and 14,15-trans-EET (1 μM each) were added to RBCs (5 × 108 RBCs in 50 μl PBS) of the vehicle control and the AUCB-treated SHR and WKY for 10 min at 37°C.
*
P < 0.05 compared with vehicle control rat RBCs for hydrolysis of the same 14,15-EET isomer. #P < 0.05 compared with the hydrolysis of 14,15-cis-EET by the same RBCs.