Fig. 2.

Both IPC and PTEN inactivation inhibit apoptosis in the reperfused myocardium. A: a TUNEL assay was performed in the hearts after they had been exposed to NIS, I-30/R-30 (I/R), or I-10/R-5 + I/R (IPC). IPC decreased apoptotic cells compared with I/R. PKO and control hearts were exposed to I/R. There were fewer apoptotic cells in PKO hearts. Arrows indicate apoptotic cells. DAPI, 4′,6-diamidino-2-phenylindole. B: percentages of TUNEL-positive cells. n = 6. *P < 0.01, I/R vs. NIS or IPC. n = 5. †P < 0.01, PKO vs. control hearts.