Fig. 1.
Effect of Lactobacillus acidophilus (LA) or Lactobacillus rhamnosus (LR) culture supernatant (CS) on P-glycoprotein (P-gp) activity and expression in Caco-2 cells. Overnight-serum-starved postconfluent Caco-2 cells were treated with a 1:50 dilution of L. acidophilus or L. rhamnosus CS in serum-free cell culture medium for 24 h. A: [3H]digoxin flux (1 μM). Results are expressed as percentage of control. Values are means ± SE of 5 separate experiments performed in triplicate. *P < 0.05 vs. untreated control. B: relative abundance of multidrug resistance 1 (MDR1) mRNA from control and treated Caco-2 cells normalized to histone mRNA (internal control). C: control and treated cell lysates were subjected to 7% SDS-PAGE and then transferred to a nitrocellulose membrane. Blot was probed with anti-MDR1 (P-gp) or anti-β-actin (β-actin) antibody. A representative blot of 3 different experiments is shown. Data were quantified by densitometric analysis and are expressed as percentage of control in arbitrary units. Values are means ± SE of 3 independent experiments performed in triplicate. *P < 0.05 vs. untreated control.