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TK6 lymphoblastoid cells and HT29 colon cancer cells were cultured with Cl-amidine in a dose-dependent manner over 24 h. A: lymphocyte cell lines undergo apoptosis in response to Cl-amidine treatment. B: colon cancer cell lines are relatively resistant to effects of Cl-amidine treatment. Apoptosis was assessed by annexin V/propidium iodide staining followed by flow cytometry. *Significant increase from control (0 μg/ml).
