Fig. 6.
Cl-amidine causes an induction of p53 in CD45+ inflammatory cells. Mice were treated with water, 1% DSS, 1% DSS + Cl-amidine (75 mg/kg ip), or 1% DSS + Cl-amidine (75 mg/kg po). p53 was induced by Cl-amidine above that of DSS alone by both routes of administration. There was a concomitant increase in the p53 target and cell cycle inhibitor p21WAF1. There was also detectable poly(ADP-ribose) polymerase (PARP) cleavage in the group treated with DSS + Cl-amidine (75 mg/kg po). Levels of protein loaded were similar, as indicated by Ponceau staining.