Fig. 1.

Effect of small-interfering RNA (siRNA)-induced knock down of occludin on filter-grown Caco-2 transepithelial electrical resistance (TER). A: siRNA occludin transfection caused a significant decrease in occludin mRNA levels as measured by real-time PCR (4 days posttransfection). *P < 0.001 vs. control. B: siRNA occludin transfection resulted in a near-complete depletion of occludin expression as assessed by Western blot analysis (6 days posttransfection). C: siRNA-induced knock down of occludin did not affect Caco-2 TER over the 6-day experimental period compared with controls.