Fig. 8.

Hypoxia and zinc induce translocation of PKC-ε in isolated pulmonary endothelial cells. Western blot analysis of PKC-ε revealed a translocation of protein from the cytoplasm to the membrane fraction following hypoxia (A–D). These changes were reversed by the addition of 10 μM TPEN. Treatment with exogenous zinc also resulted in a significant loss of PKC-ε in the cytoplasm and accumulation in the membrane fraction (E–G) that was reversed by TPEN (10 μM). Protein levels were normalized to β-actin (n = 3, *P < 0.05, **P < 0.001).