Fig. 2.

CSE suppresses IAV-stimulated cytokine and retanoic acid-inducible gene (RIG)-I mRNA expression in the human lung. Human lung slices were exposed to 6 × 10⁶ PFU/ml of IAV PR8 in the presence or absence of CSE for 24 h. Total RNA was then isolated from lung slices. Relative end-point RT-PCR products were separated by agarose gel electrophoresis, and mRNA expression was determined by densitometry of the appropriate bands on ethidium bromide-stained gels. Transcript levels of cytokines (A), IFNs (B), and RIG-I (C) were normalized relative to the constitutively expressed GAPDH gene. Data are representative of 3 separate experiments. *Lanes were not adjacent but came from the same original gel.