Table 3.
Overall effect of pharmacologic or genetic disruptions of the transient receptor potential vanilloid 1 on mortality, cytokine gene expression and serum nitrate/nitrite levels after cecal ligation and puncture or lipopolysaccharide challenge*
| Cecal Ligation and Puncture | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Perturbation | Mortality | Blood Culture | Cytokine gene expression in splenocytes | Serum levels | |||||||
| (168 h) | Colony forming units/ml | TNFα Time (h) | CCL3 Time (h) | IL-10 Time (h) | Nitrate/Nitrite Time (h) | ||||||
| Pharmacologic | 6 | 24 | 6 | 24 | 6 | 24 | 6 | 24 | 6 | 24 | |
| WT+RTX | ↑ | → | ↑ | → | ↓ | → | → | → | → | ↑ | → |
| Genetic | |||||||||||
| Trpv1-KO | ↑ | → | → | → | → | → | ↑ | → | ↑ | → | → |
| Trpv1-KO+RTX | → | ||||||||||
| Lipopolysaccharide Challenge | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Perturbation | Mortality | Cytokine gene expression in splenocytes | Serum levels | ||||||||||
| (168 h) | TNFα Time (h) | CCL3 Time (h) | IL-10 Time (h) | Nitrate/Nitrite Time (h) | |||||||||
| Pharmacologic | 3 | 6 | 24 | 3 | 6 | 24 | 3 | 6 | 24 | 3 | 6 | 24 | |
| Capsazepine | ↑ | → | → | ↑ | → | → | → | ↓ | ↑ | → | ↑ | → | → |
| Resiniferatoxin | → | → | → | → | → | → | → | → | → | → | → | → | → |
| Genetic | |||||||||||||
| Trpv1-KO | → | → | → | → | → | → | → | → | → | ↑ | → | → | → |
*
Arrows pointing up or down indicate statistically significant effects (in the direction of the arrow) of given disruptions compared to respective controls and arrows pointing to the right indicate no significant effect. Cells were left empty when measurements were not obtained. CCL3 indicates macrophage inflammatory protein-1 α, IL-10 interleukin 10, RTX resiniferatoxin, TNFα tumor necrosis factor, Trpv1-KO animals lacking the transient receptor potential vanilloid 1gene, and WT wild-type.