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. Author manuscript; available in PMC: 2012 Jun 3.
Published in final edited form as: Biochem Biophys Res Commun. 2011 May 8;409(2):338–343. doi: 10.1016/j.bbrc.2011.05.020

Fig. 2. Identification of the interaction domains of TBX20b and MKLN1.

Fig. 2

(A) Top: Schematic of TBX20 isoforms. Bottom: COSM6 cells were transfected with HA-MKLN1 and GST, GST-TBX20a, GST-TBX20b, and truncations of GST-TBX20b. Copurification assays and western blot analyses compared whole cell lysate (WCL) and the elution fraction (EF). The blots were probed with GST and HA1.1 antibodies as before. (B) Top: Schematic of MKLN1 protein. Bottom: Similar copurification analyses were performed with truncated HA-tagged MKLN1 constructs. Gels were loaded as in panel (A) comparing WCL and EF with the GST and the GST-TBX20b transfected cells. Western blot analysis of the truncated HA-MKLN1 constructs (top-to-bottom) are listed left-to-right.