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. Author manuscript; available in PMC: 2012 Jun 8.
Published in final edited form as: Cell Metab. 2011 Jun 8;13(6):690–700. doi: 10.1016/j.cmet.2011.04.007

Figure 1. Inactivation of the Ptpmt1 gene leads to mouse embryonic lethality.

Figure 1

A) Gene targeting strategy. Exons (blue boxes), translation initiation site (ATG), selection gene (neomycin, neo), positions of 5′ and 3′ probes for Southern blot (red bars), PCR primer (red arrows), loxP sites ( ), Frt sites ( ), and restriction sites are indicated. X-XbaI, E-EcoRI. B) Genotype of offspring from Ptpmt1 heterozygous intercrosses at various developmental stages. C) Derivation of Ptpmt1-deficient mouse embryonic fibroblasts. Ptpmt1flox/flox MEFs were infected with recombinant adenoviruses encoding GFP(−) or GFP-Cre (+). Cells were harvested at the indicated time points post-infection for western blot analysis. dpi, days post infection. See also Figure S1.