Fig. 4.

Doxorubicin-induced suppression of LC differentiation is reversible. hTERT-MUTZ3 cells, dox90+ cells, and dox90− cells (drug-free) were differentiated into immature LC for 10 days after 1, 3, and 4 months of drug depletion of the dox90− cells. a dotplots showing CD1a and Langerin expression on hT-iLC, dox90+ iLC, and dox90− iLC. Upon a drug-free period of at least 3 months, the dox90− cells regained their differentiation potential. b Percentages of cells expressing the DC maturation marker CD83 in cultures started 1, 3, and 4 months after drug depletion of the dox90− cells. c MLR was performed using hT-mLC, dox90+ mLC, and dox90− mLC [depleted for drugs for 1 month (left), 3 months (middle), and 4 months (right)], to analyze the T-cell stimulatory capacities of the mLC