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. 2011 Feb;30(1):19–28. doi: 10.1089/hyb.2010.0083

FIG. 3.

FIG. 3.

FB11 binds with equal intensity to all rungs of the LPS ladder, whereas the binding intensity of the other three anti-OAg IgG2a MAbs decreases with decreasing chain size. (A) Western blot analysis of the indicated MAbs, at the indicated concentrations, on LVS lysate. The positions of pre-stained molecular weight standards (in kDa) are indicated. (B) Model of antibody binding (only one antibody arm shown) to terminal or internal OAg epitopes for the lower rungs of the LPS ladder in the Western blot. Schematic of Ft LPS and the four sugar repeating unit of OAg is shown at top. QNF, QuiN4Fm, 4,6-dideoxy-4-formamido-D-glucose; GNA, GalNAcAN, 2-acetamido-2-deoxy-D-galacturonamide; QN, QuiNAc, 2-acetamido-2,6-dideoxy-D-glucose.(5,6) The higher sensitivity and different laddering pattern of FB11 compared with the other three IgG2a anti-Ft OAg MAbs were observed on numerous Western blots.