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. 2011 Feb;30(1):19–28. doi: 10.1089/hyb.2010.0083

FIG. 6.

FIG. 6.

The affinity of FB11, but not of Ab52, can be determined using immobilized MAb and soluble Ft OAg. (A) Biacore analysis of the binding of immobilized FB11 (top) and Ab52 (bottom) to Ft OAg. MAbs were captured on anti-mouse IgG-coated chips and probed with Ft OAg. (B) Schematic of the monovalent binding of immobilized FB11 to Ft OAg (top) and of the multivalent binding of immobilized Ab52 to Ft OAg (bottom).