Lateral views of wildtype and prdm1a mutant embryos at 25hpf embryos. col9a2 is expressed in the posterior pharyngeal arches, posterior to the otic vesicle (A) and within the notochord (C) in wild type embryos. col9a2 is upregulated in the posterior pharyngeal arches, otic vesicle (B) and slightly within the notochord (D) of prdm1a mutant embryos. agr2 is modestly expressed within the hatching gland and otic vesicle of wild type embryos (E), but is dramatically upregulated in the hatching gland of prdm1a mutant embryos (F). e, eye; hg, hatching gland.