TABLE 3.
Distribution of Neurons Expressing CRF1-LI in the Mouse Hindbrain: Density and Intensity
| Region | Density1 | Intensity2 |
|---|---|---|
| Superior colliculus | ++ | +/++3 |
| Inferior colliculus | ++ | +/++ |
| Tegmental nuclei | +++ | ++/+++ |
| Pedunculopontine nucleus | +++ | +++ |
| Red nucleus | ++++4 | +++ |
| Substantia nigra | ||
| Pars compacta | ++ | +++ |
| Pars reticulata | ++ | ++/+++ |
| Raphe nuclei | ||
| Dorsal nucleus | ++ | +++/++++ |
| Median nucleus | + | ++ |
| Edinger-Westphal nucleus | +++ | +/++ |
| Principal oculomotor nucleus |
+++ | +++ |
| Darkschewitsch nucleus | ||
| Nuclei of the trigeminal nerve |
||
| Mesencephalic nucleus | ++++ | +++ |
| Motor nucleus | ++++4 | +++ |
| Principal sensory nucleus |
+++ | +/++ |
| Spinal nucleus | ++ | +/++ |
| Facial nucleus | ++++4 | +++ |
| Vestibular nuclei | +++ | ++/+++ |
| Cochlear nuclei | ||
| Dorsal nucleus | ++ | ++ |
| Ventral nucleus | ++ | +++ |
| Vagus nucleus | ++++ | ++++ |
| Hypoglossal nucleus | ++++ | +++ |
| Solitary nucleus | +++ | +/++ |
| Ambiguus nucleus | +++ | ++++ |
| Locus coeruleus | +++ | ++++ |
| Superior olive | +++ | +++ |
| Inferior olive | +++ | ++/+++ |
| Nuclei of lateral lemniscus |
+++ | +++ |
| Nucleus of trapezoid body | ||
| Medial nucleus | ++++ | ++++ |
| Lateral nucleus | +++ | ++ |
| Cerebellum | ||
| Molecular layer | +++ | + |
| Purkinje cell layer | ++++ | ++++ |
| Granular cell layer | +++ | + |
| Cerebellar nuclei | +++ | +++ |
1
Density values are based on the percentage of positive cells related to total cell number. −, no positive cells; −/+, occasional cells, <10%; +, low, 10–5%; ++, moderate, 25–50%; +++, dense, 50–75%; ++++, very dense, >75%.
2
Staining intensity: +, weakly positive; ++, moderately positive; +++, intensely positive; ++++, strongly intense.
3
Two scores are noted because the region contains cell populations with different labeling intensity.
4
All large, multipolar neurons were CRF1 immunoreactive.