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LSECs from fibrotic livers are immunogenic. Freshly isolated LSECs (2 × 105) from normal or fibrotic livers were either A, loaded with OVA257–264 and cocultured with CD8+ OT-I T cells (2 × 105) before measurement of T cell activation markers or B, coincubated with CD4+ T cells (2 × 105) from C57BL/6 mice before determination of CD25 and Foxp3 coexpression. Data are representative of experiments repeated three times.
