FIGURE 6.

F-LSECs produce enhanced T cell proliferation in vivo. Mice were adoptively transferred with OT-I T cells (2 × 10⁶) before receiving footpad immunization with LSEC. OVA₂₅₇ (2 × 10⁵) or mock immunized. At 96 h, ipsilateral popliteal lymph nodes were harvested and costained for CD8 and OVA tetramer. The fraction of OVA tetramer⁺ cells among all CD8⁺ cells (A), the total number of Ova tetramer⁺ cells (B), and their expression of CD44 (C) were determined by flow cytometry. Experiments were repeated three times using two to four mice per experiment. *p < 0.05.