FIGURE 9.

F-LSECs do not mitigate Ag presentation by DC. OT-I T cells (1 × 10⁵) were cultured alone or coincubated with DC.OVA_257–264 (1 × 10⁴) and LSECs (1 × 10⁴), separately or in combination, harvested from normal or fibrotic livers. DC.OVA₂₅₇ induced potent Ag-specific T cell proliferation that was mitigated by coculture with N-LSECs. Conversely, coculture with F-LSECs did not lower DC-mediated T cell proliferation. Experiments were repeated three times and performed in triplicate. *p < 0.05.