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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biomaterials. 2011 May 5;32(23):5380–5390. doi: 10.1016/j.biomaterials.2011.04.008

Figure 5.

Figure 5

Immediate response of fibroblast in ETs – (A) Percent viability, (B) a typical fluorescence micrograph of NORM group showing all Hoechst stained cells, and (C) corrected viability. Terminal interface location, where the freezing interface stopped at the end of the freezing protocol, is noted with a dashed line. In the frozen/thawed region (i.e., x < terminal interface location), the viability of all groups decreases with increasing distance from the interface. Without considering the decrease in the cell number, the highest viability is noted in the HCEL group. However, the fluorescence micrograph shows significant decreased cell number in the frozen/thawed region. If the decrease in the cell number is considered in calculating the cell viability, the highest (corrected) viability is observed in the HCOL group.