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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Biomaterials. 2011 May 5;32(23):5380–5390. doi: 10.1016/j.biomaterials.2011.04.008

Figure 6.

Figure 6

Fluorescence micrographs of cytoskeleton of fibroblasts in the frozen/thawed and unfrozen regions – (A) Overall appearance of actin cytoskeleton of fibroblasts (scale bar = 100 μm), and (B) close-up images of actin cytoskeleton (scale bar = 20 μm). In both the NORM and HCEL groups, the cytoskeleton in the frozen/thawed regions is damaged, with shortened extensions and fewer number of cells. In the HCOL group, the cells have condensed and thick actin bundles, and less damage is observed after F/T than the NORM and HCEL groups.