. Author manuscript; available in PMC: 2011 Aug 1.

Published in final edited form as: J Am Soc Mass Spectrom. 2010 Apr 24;21(8):1432–1445. doi: 10.1016/j.jasms.2010.04.004

Table 1.

Summary of Identified Cross-linked Peptides of Cytochrome C after Enrichment.

Type	Peptide Sequence	AA location	m/z(Observed)	z	Δ(ppm)	Seen Before Enrichment	References
0	Ac-GDVEKGK	G1-K7	712.3342	2	5	Yes
0	Ac-GDVEKGKK	G1-K8	517.9262	3	9	Yes
0	KIFVQK	K8-K13	706.3799	2	7	Yes	40,41,67
0	GGKHK	G23-K27	588.2895	2	6	Yes
0	KTGQAPGFSYTDANK	K39-K53	745.3507^*	3	7	Yes	40,41,66,67
0	KTGQAPGFSYTDANK	K39-K53	559.2618	4	2	Yes	40,41,66,67
0	KTGQAPGFSYTDANKNK	K39-K55	826.0621	3	5	Yes	40,65,66,67
0	KTGQAPGFSYTDANKNK	K39-K55	619.7963	4	1	Yes	40,65,66,67
0	KYIPGTK	K73-K79	728.3737	2	5	Yes	41,67
0	KYIPGTK	K73-K79	485.9183	3	5	Yes	41,67
0	KGER	K88-R91	569.7707	2	1	Yes
0	EDLIAYLKK	E92-K100	871.4472	2	1	Yes	67
0	EDLIAYLKK	E92-K100	581.3033	3	5	Yes	67
0	KATNE	K100-E104	606.2740	2	2	Yes
1	Ac-GDVEKGKK	G1-K8	767.3693	2	−5	No
1	Ac-GDVEKGKKIFVQK	G1-K13	717.0482	3	7	Yes
1	GKKIFVQK	G6-K13	789.9327	2	6	Yes	41,66,67
			526.957^*	3	5	Yes
			395.4689	4	3	Yes
1	GGKHKTGPNLHGLFGR	G23-R38	769.7238	3	−1	Yes	8,40,41,66,67
			577.5500^*	4	8	Yes
			462.2401	5	5	Yes
1	GGKHKTGPNLHGLFGRK	G23-K39	609.5687	4	−1	Yes	66,67
			487.8605	5	7	Yes
			406.7162	6	3	Yes
1	KTGQAPGFSYTDANKNK	K39-K55	1229.5850	2	6	Yes	8,40,41,66,68
			820.0587	3	5	Yes
			615.2941	4	2	Yes
1	MIFAGIKKK	M80-K88	833.9532	2	10	Yes	8,40,41,66,67
1	MIFAGIKKK	M80-K88	556.2999	3	1	Yes	8,40,41,66,67
1	M_oxIFAGIKKK	M80-K88	841.9451	2	3	Yes
1	M_oxIFAGIKKK	M80-K88	561.6340^*	3	5	Yes
1	KKGER	K87-R91	624.8149	2	4	Yes	41
1	KKGER	K87-R91	416.8803	3	6	Yes	41
1	GEREDLIAYLKKATNE	G89-E104	827.7458	3	5	Yes
1	GEREDLIAYLKKATNE	G89-E104	621.0619	4	6	Yes
1	EDLIAYLKKATNE	E92-E104	1070.0338	2	7	Yes	67
1	EDLIAYLKKATNE	E92-E104	713.6908	3	6	Yes	67
2	Ac-GDVEKGK KIFVQK	G1-K7 ~ K8-K13	723.0504	3	6	Yes	3,40,41,67
2	Ac-GDVEKGK KIFVQK	G1-K7 ~ K8-K13	542.5392^*	4	5	Yes	3,40,41,67
2	Ac-GDVEKGK KK	G1-K7 ~ K87-K88	560.6229	3	6	Yes	67,68
2	Ac-GDVEKGK KK	G1-K7 ~ K87-K88	420.7197	4	7	Yes	67,68
2	Ac-GDVEKGK KKGER	G1-K7 ~ K87-R91	506.2615	4	7	Yes	67,68
2	Ac-GDVEKGKK KK	G1-K8 ~ K87-K88	452.7451	4	10	Yes	67
2	Ac-GDVEKGKK KKGER	G1-K8 ~ K87-R91	538.2844	4	5	Yes	67,68
2	GKK KGER	G6-K8 ~ K88-R91	726.3632	2	−17	Yes
2	GKK KATNE	G6-K8 ~ K100-E104	762.8826^*	2	6	Yes	66,68
			508.9219	3	1	Yes
			381.9450	4	5	Yes
2	KIFVQK KATNE	K8-K13 ~ K100-E104	652.3468	3	9	Yes
2	KIFVQK KATNE	K8-K13 ~ K100-E104	489.5106	4	6	Yes
2	GGKHK KATNE	K23-K27 ~ K100-E104	573.6183^*	3	6	Yes
2	KTGQAPGFSYTDANK MIFAGIKKK	K39-K53 ~ M80-K88	813.4261	4	13	No
2	KTGQAPGFSYTDANK MIFAGIKKK	K39-K53 ~ M80-K88	650.9429	5	13	No
2	KTGQAPGFSYTDANK KATNE	K39-K53 ~ K100-E104	695.0784	4	2	Yes	67,68
2	KKGER KATNE	K87-R91 ~ K100-E104	453.2339	4	11	Yes
2	KGER KATNE	K88-R91 ~ K100-E104	561.2752	3	7	Yes
2	KATNE KATNE	K100-E104 ~ K100-E104	585.6121	3	10	Yes

Note: All of the cross-linked peptides have displayed characteristic losses in MS/MS.

These cross-linked peptides have been further confirmed by MS³.